Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Regulation of tamoxifen sensitivity by the PLAC8/MAPK pathway axis is antagonized by curcumin-induced protein stability change

doi: 10.1007/s00109-021-02047-5

Figure Lengend Snippet: Relationship between the ubiquitin of PLAC8 and curcumin. a Western blot (upper) tested the expression level of PLAC8 decreased by curcumin in a dose- and time-dependent manner. RT-PCR (bottom) showed the mRNA expression level of PLAC8 was not changed by curcumin. The expression was quantified and normalized to GAPDH. b Western blot tested the expression level of PLAC8 decreased by treatment of curcumin 24 h (5 μM) could be recused by treatment of MG-132 6 h (10μM) in MCF-7/TAM (upper) and MCF-7 (bottom). c MCF-7/TAM cells were transfected with PLAC8. At 24 h post transfection, cells were treated with or without curcumin (5 μM). After 18 h later, cells were treated with or without MG132 (10 μM) for 6 h and immunoprecipitated with PLAC8 antibody. d Cell proliferation assay showed that curcumin (10 μM) alone or combined with MAPKs inhibitors (SCH772984 10 μM or SB2020190 20 μM) in MCF-7/TAM 24hr. e MCF-7/TAM treated with curcumin (10 μM) alone or combined with MAPKs inhibitors(SCH772984 10 μM or SB2020190 20 μM) and then overexpressing PLAC8 expression. All data represent mean ± SD of three experiments performed in triplicate. ** p < 0.01; * p < 0.05; NS, not significant

Article Snippet: The slices were stained with PLAC8 (1:200) and Ki-67 (1:500) (100130-MM22, Sino Biological, Beijing, China) for 1 h at 37 °C.

Techniques: Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Transfection, Immunoprecipitation, Proliferation Assay

Journal: Journal of Molecular Medicine (Berlin, Germany)

Article Title: Regulation of tamoxifen sensitivity by the PLAC8/MAPK pathway axis is antagonized by curcumin-induced protein stability change

doi: 10.1007/s00109-021-02047-5

Figure Lengend Snippet: Tamoxifen inhibited tumor growth and showed a synergistic activity with curcumin in vivo. ( a ) Body weight ( b ), tumor volume (every 3 days) ( c ) and tumor weight (day 24 after treating drug) ( d ) of xenograft-bearing mice in four groups. 1, Control; 2, Curcumin; 3, Tamoxifen; 4, Curcumin and Tamoxifen. ( ∗* P < 0.01 vs. control group [upper], # P < 0.05 vs. single-treated group). e Western blot analysis results showed that curcumin induced the downregulation of PLAC8 protein levels in MCF-7/TAM xenograft-bearing mice. f IHC staining showed the expression of Ki67 and PLAC8. Scale bar represents 50 μm. g Hypothesis model of curcumin action on regulating BC progression. Our indicated that PLAC8 could induce the tamoxifen resistance, thus affecting the ERK1/2-p38 kinase pathways. In addition, curcumin decreased the level of PLAC8 through ubiquitination modification. This mechanism may explain the potential mechanism of tamoxifen resistance to breast cancer

Article Snippet: The slices were stained with PLAC8 (1:200) and Ki-67 (1:500) (100130-MM22, Sino Biological, Beijing, China) for 1 h at 37 °C.

Techniques: Activity Assay, In Vivo, Western Blot, Immunohistochemistry, Expressing, Modification

Journal: Journal of Cellular and Molecular Medicine

Article Title: Bioenergetic modulators hamper cancer cell viability and enhance response to chemotherapy

doi: 10.1111/jcmm.13642

Figure Lengend Snippet: In vivo effect of BM s in U251 glioma microtumour growth. Representative pictures (16× [in ovo] and 12.5× [ex ovo] magnifications) of BM s effect on the perimeter and in vascularization of tumours, after 4 days of treatment in and ex ovo. Tumour growth was measured in ovo, and blood vessels around the tumours were counted ex ovo. Immunohistochemical analysis of Ki67 expression in treated tumours was compared with treated group (200× magnification). Pictures are representative of n = 20 eggs. *** P < .001 compared to untreated cells

Article Snippet: Immunohistochemistry for Ki67 (AP10243CM, Gennova) was performed according to the avidin‐biotin‐peroxidase principle (R.T.U.

Techniques: In Vivo, In Ovo, Immunohistochemical staining, Expressing

Journal: eLife

Article Title: Homeostatic and tumourigenic activity of SOX2+ pituitary stem cells is controlled by the LATS/YAP/TAZ cascade

doi: 10.7554/eLife.43996

Figure Lengend Snippet:

Article Snippet: Antibody , Mouse monoclonal anti-p63 , A Menarini Diagnostics , Cat# MP163 , IHC 1:100.

Techniques: Blocking Assay, Plasmid Preparation, BIA-KA, Western Blot, Software